By definition
\begin{equation}
\text{int} \langle U \rangle : = \bigcup \{ V \in \mathcal{T} : V \subseteq U \}
\end{equation}
Result
R125: Subset relation is reflexive shows that $U$ itself satisfies $U \subseteq U$. Since $U$ is also an open set, then $U$ is a set in the union $\text{int} \langle U \rangle$ and result
R4142: Union is an upper bound to each set in the union implies that $U \subseteq \text{int} \langle U \rangle$.
Conversely, result
R3945: Set is a superset to its interior guarantees that $\text{int} \langle U \rangle \subseteq U$. Since we have inclusions in both directions, the claim then follows as a consequence of
R2741: Set equality iff inclusion in both directions. $\square$